Common lab protocols
· Calcium Phosphate Transfection
Electroporation
· General Electroporation settings
· Bulk electroporation in vivo
· Arseny’s Electrophysiology Solutions
Histochemistry
· Zahraa’s Immunostaining protocol
· Fish gelatin fixation_for_cryosectioning
· OCT cryosectioning and staining protocol
· PFA Fixation for cryosections
· Dent's Fixation for cryo sections
Husbandry
· NASCO injection schedule
· Tadpole Chow for rearing to late stages
· Standard subcloning procedure
· Standard PCR protocol for subcloning
· Transformation of competent cells
· Phenol/chloroform extraction
· Miniprep by ethanol precipitation
· 10x PBS
· SDSPAGE
· Using SolidworksPro 3D modeling software
Imaging
· 2P microscope laser alignment