Common lab protocols

 

Cell Culture

·      Tectal cell culture

·      Calcium Phosphate Transfection

·      EphA3-AP protocols

 

Electroporation

·      General Electroporation settings

·      Bulk electroporation in vivo

·      Single-cell electroporation

 

Electrophysiology

·      Drugs for electrophysiology

·      Cline Internal & External

·      Arseny’s Electrophysiology Solutions

 

Histochemistry

·      Basic immuno protocol

·      Zahraa’s Immunostaining protocol

·      DAPI Staining protocol

·      Paraformaldehyde

·      Wholemount immuno standard

·      Fish gelatin fixation_for_cryosectioning

·      OCT cryosectioning and staining protocol

·      PFA Fixation for cryosections

·      Dent's Fixation for cryo sections

·      Immunofluorescence(FG)

 

Husbandry

·      Xenopus husbandry

·      Zebrafish husbandry

·      NASCO injection schedule (defunct)

·      Oocyte procedures

·      Media for frog experiments

·      MBSH buffer

·      Tadpole Chow for rearing to late stages

·      In Vitro Fertilization

·      Sperm Nucleus Injection

 

Molecular Biology

·      Standard subcloning procedure

·      Standard PCR protocol for subcloning

o   PCR Best Practices

·      Standard Restriction Digest

·      Transformation of competent cells

·      Phenol/chloroform extraction

·      Total RNA Isolation

o   RNA Work Best Practices

·      Miniprep by ethanol precipitation

·      Sequencing

·      10x PBS

·      Brain_extracts_from_Xenopus

·      Electrophoresis_buffers

·      Western Blotting

·      SDSPAGE

 

Software

·      Using SolidworksPro 3D modeling software

 

Imaging

·      2P microscope laser alignment

 

Documents

·      Lab_order_form.doc

·      Plasmid Inventory Sheet