LAB PROTOCOLS:

 

Cell Culture

Tectal cell culture

Calcium Phosphate Transfection

EphA3-AP protocols

 

Electroporation

Electroporation settings

 

Electrophysiology

Drugs

Cline Internal & External

Xenopus Electrophysiology Solutions

 

Histochemistry

Basic immuno protocol

DAPI Staining protocol

Paraformaldehyde

Wholemount immuno standard

Fixation_for_cryosectioning

 

Husbandry

Detailed Frog Breeding Protocol (current)

NASCO injection schedule

Mare-Human gonadotropin injection schedule

Benoit's Frog Breeding Schedule

Oocyte procedures

Media for frog experiments

MBSH buffer

Tadpole Chow

 

Imaging

Using SolidworksPro 3D modeling software


Molecular Biology

Standard subcloning procedure

Standard PCR protocol for subcloning

Standard Restriciton Digest

Phenol/chloroform extraction

Total RNA Isolation

Miniprep by ethanol precipitation

Sequencing

10x PBS

Brain_extracts_from_Xenopus

Electrophoresis_buffers

SDSPAGE

PFA Fixation for cryosections

Dent's Fixation for cryo sections

Immunofluorescence(FG)

 

Storage

Lab_order_form.doc

Plasmid Inventory Sheet