_____________________________________________ ______/______/______
________µm sections in
_____________________plane
1.
Incubate 2 x 20 min. in 0.1M PB at R.T. o o
2.
Incubate 4 x 10 min in 0.1M
PB + 0.3% triton at R.T. o o o
o
3.
Incubate 30 min in 0.1M PB + 0.3% triton +5% NGS at RT o
4.
Incubate overnight with
primary antibody _______________
Diluted
_____________ in 0.1M PB + 0.03% triton at 40C. o
5. Rinse
4 x 10 min in 0.1M PB + 0.3% triton at R.T. o o o o
6.
Incubate slides 1 hr in
secondary antibody _______________
Diluted
_____________ in 0.1M PB + 0.03% triton at R.T. o
7. Rinse
3 x. 10 min in 0.1M PB at R.T. o o o
8. 3
drops mounting medium + coverslip.
Store at Ð200C