Immunohistochemistry protocol

 

 

_____________________________________________    ______/______/______

 

 

________m sections in _____________________plane

 

1.  Incubate 2 x 20 min. in 0.1M PB at R.T.                                  o   o

2.  Incubate 4 x 10 min  in 0.1M PB + 0.3% triton at R.T.   o   o   o   o

3.  Incubate 30 min in 0.1M PB + 0.3% triton +5% NGS at RT o

4.      Incubate overnight with primary antibody _______________

Diluted _____________ in 0.1M PB + 0.03% triton at 40C. o

5.  Rinse 4 x 10 min in 0.1M PB + 0.3% triton at R.T.                                                                                                                           o   o   o   o

6.      Incubate slides 1 hr in secondary antibody _______________

Diluted _____________ in 0.1M PB + 0.03% triton at R.T.            o

7.  Rinse 3 x. 10 min in 0.1M PB at R.T.                                      o    o    o

8.  3 drops mounting medium + coverslip.  Store at 200C