Electrophoresis buffers and
solutions |
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STOCKS: |
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1.5M Tris/HCl pH8.8 |
90.75g/500ml |
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0.5M Tris/HCl pH6.8 |
30.25g/500ml |
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--autoclave both solutions |
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20% SDS |
100g/500ml |
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use complete
100g container, so you don’t have to weight the powder, |
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powder very harmful!!!, prepare it under the hood |
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10% APS |
1g/10ml, keep at 4oC |
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TEMED |
ready to use solution, keep at 4oC |
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Acrylamide |
40% ( 37.5/1) ready to use, keep at 4 oC |
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acrylamide, bisacrylamide crystals very toxic, |
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better to buy ready to use solution |
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Tris/Glycine Running Buffer |
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1x |
5x/1L |
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Tris base |
25mM |
15.0g |
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Glycine |
192mM |
72g |
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SDS |
0.10% |
25ml 20% |
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Transfer Buffer (Blotting Buffer) |
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1x |
4L |
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Tris base |
48mM |
23.2g |
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Glycine |
39mM |
11.65g |
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SDS |
0.037% |
7.4ml 20% |
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Methanol |
20% |
800mL |
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add methanol after dissolving salts, keep at 4C |
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Sample Buffer - Laemmli Buffer |
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1x |
2x/20mL |
4x/10mL |
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Tris/HCl pH6.8 |
50mM |
4mL 0.5M |
4mL 0.5M |
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Glycerol |
10% |
4g |
4g |
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SDS |
2% |
4ml 20% |
0.8g |
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Bromphenol blue |
0.025% |
0.01g |
0.01g |
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DTT |
100mM |
0.6g |
0.6g |
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store sample buffer at -20C |
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