Electrophoresis buffers and solutions
STOCKS:
1.5M Tris/HCl pH8.8	90.75g/500ml
0.5M Tris/HCl pH6.8	30.25g/500ml
--autoclave both solutions
20% SDS	100g/500ml
	use complete 100g container, so you don’t have to weight the powder,
	powder very harmful!!!, prepare it under the hood
10% APS	1g/10ml, keep at 4oC
TEMED	ready to use solution, keep at 4oC
Acrylamide	40% ( 37.5/1) ready to use, keep at 4 oC
	acrylamide, bisacrylamide crystals very toxic,
	better to buy ready to use solution
Tris/Glycine Running Buffer		1x	5x/1L
	Tris base	25mM	15.0g
	Glycine	192mM	72g
	SDS	0.10%	25ml 20%
Transfer Buffer (Blotting Buffer)		1x	4L
	Tris base	48mM	23.2g
	Glycine	39mM	11.65g
	SDS	0.037%	7.4ml 20%
	Methanol	20%	800mL
	add methanol after dissolving salts, keep at 4C
Sample Buffer - Laemmli Buffer		1x	2x/20mL	4x/10mL
	Tris/HCl pH6.8	50mM	4mL 0.5M	4mL 0.5M
	Glycerol	10%	4g	4g
	SDS	2%	4ml 20%	0.8g
	Bromphenol blue	0.025%	0.01g	0.01g
	DTT	100mM	0.6g	0.6g
	store sample buffer at -20C