Xenopus buffer
MBS-H (Modified Barth
solution) 100x/1l
88mM NaCl 51.4g
1mM KCl 0.75g
2.4mM NaHCO3 2.0g
0.82mM MgSO4
x6H2O 1.66g
0.33mM Ca(NO3)2
x4H2O 0.78g
0.41mM CaCl2 x2H2O 0.6g
10mM HEPES 23.8g
400U/ml Streptomycin /Penicillin 2.5ml Strep/Pen 10000U/ml
adjust pH to 7.4 with NaOH
sterile filter buffer
NOTE: The working dilution of MBS-H is 0.1x. This means that the 10x stock should be
diluted 100x to get the final working concentration.