SDS-PAGE

 

 All volumes in ml.  Total 10 ml = for 2 minigels

 

 

5%

6%

7%

8%

9%

10%

12%

15%

40% acrylamide

 

1.5 M Tris Cl pH 8.8

 

20% SDS

 

H2O

 

10% APS

 

Temed

1.25

 

2.5

 

0.05

 

6.1

 

0.1

 

0.01

1.5

 

2.5

 

0.05

 

5.85

 

0.1

 

0.01

1.75

 

2.5

 

0.05

 

5.6

 

0.1

 

0.01

2

 

2.5

 

0.05

 

5.35

 

0.1

 

0.01

2.25

 

2.5

 

0.05

 

5.1

 

0.1

 

0.01

2.5

 

2.5

 

0.05

 

4.85

 

0.1

 

0.01

3

 

2.5

 

0.05

 

4.35

 

0.1

 

0.01

3.8

 

2.5

 

0.05

 

3.6

 

0.1

 

0.01

 

Stacking gel

 

40% acrylamide                            0.94                prepare stock solution of stacking gel without APS and TEMED

0.5 M Tris Cl pH 6.8                 2.5                  

20% SDS                                            0.05                take 2.5ml of stock solution/gel, add 25Ál APS, 2.5Ál TEMED

H2O                                                       6.4

10% APS                                           0.1

Temed                                                  0.01