All volumes in ml.
Total 10 ml = for 2 minigels
|
5% |
6% |
7% |
8% |
9% |
10% |
12% |
15% |
40% acrylamide 1.5 M Tris Cl pH 8.8 20% SDS H2O 10% APS Temed |
1.25 2.5 0.05 6.1 0.1 0.01 |
1.5 2.5 0.05 5.85 0.1 0.01 |
1.75 2.5 0.05 5.6 0.1 0.01 |
2 2.5 0.05 5.35 0.1 0.01 |
2.25 2.5 0.05 5.1 0.1 0.01 |
2.5 2.5 0.05 4.85 0.1 0.01 |
3 2.5 0.05 4.35 0.1 0.01 |
3.8 2.5 0.05 3.6 0.1 0.01 |
40% acrylamide 0.94 prepare
stock solution of stacking gel without APS and TEMED
0.5 M Tris Cl pH 6.8 2.5
20% SDS 0.05 take
2.5ml of stock solution/gel, add 25µl APS, 2.5µl TEMED
H2O 6.4
10% APS 0.1
Temed 0.01