Miniprep Protocol 12/21/00
Grow one colony in 3ml LB + antibiotic overnight (>6hr) at 37C shaking
1. transfer 1.5ml to eppendorf
2. Spin 8000rpm x 3 min
3. discard supernatant
4. resuspend pellet in 100ul of Resuspension Solution
5. (incubate 5 min at rt)
6. add 200ul cell lysis solution, mix by inverting
7. (incubate 5 min on ice)
8. add 150ul Neutralization Solution and vortex
9. (incubate 5 min on ice)
10. spin max rpm x 3 min
11. take 350ul of supernatant and transfer to fresh eppendorfs
12. add 700ul EtOH
13. incubate 2 min at RT
14. spin max rpm x 3 min
15. spill out supernatant and gently apply 70% EtOH wash.
16. spill out supernatant gently.
17. dry eppendorfs by suspending upsidedown on a paper towel in incubator
18. resuspend pellet in 30-40ul ddH2O and keep on ice.