Electroporator settings:

For Xenopus:

Brain or eye electroporation:

Make sure to use capacitors and set output to MONO

DNA moves toward positive pole.

DURATION 1.6ms

VOLTAGE 30-70V (higher settings may kill animals)

Single cell electroporation/iontophoresis of dextans or DiI:

Do not use capacitors

Set output to MONO - pipette should be + lead and bath should be - lead

DURATION 120ms

VOLTAGE 30-80V

Single cell electroporation/iontophoresis of DNA:

Do not use capacitors

Set output to MONO - pipette should be - lead and bath should be + lead

FREQUENCY 200Hz

DURATION 1ms

VOLTAGE 30-80V (too high will clog tip)

Give 3-5 bursts of about 1 sec each. (REPEAT not SINGLE button)