Electroporator settings:

 

For Xenopus:

 

Brain or eye electroporation:

Make sure to use capacitors  and set output to MONO

                  DNA moves toward positive pole.

                  DURATION                       1.6ms

                  VOLTAGE                          30-70V (higher settings may kill animals)

 

 Single cell electroporation/iontophoresis of dextans or DiI:

                  Do not use capacitors

                  Set output to MONO  -  pipette should be + lead and bath should be - lead

                  DURATION                       120ms

                  VOLTAGE                          30-80V

 

Single cell electroporation/iontophoresis of DNA:

                  Do not use capacitors

                  Set output to MONO  -  pipette should be -  lead and bath should be + lead

                  FREQUENCY                  200Hz

                  DURATION                       1ms

                  VOLTAGE                          30-80V (too high will clog tip)

                  Give 3-5 bursts of about 1 sec each. (REPEAT not SINGLE button)