Electroporator settings:
For Xenopus:
Brain or eye electroporation:
DNA
moves toward positive pole.
DURATION 1.6ms
VOLTAGE 30-70V (higher settings may kill animals)
Single cell
electroporation/iontophoresis of dextans or DiI:
Do not use capacitors
Set output to MONO - pipette should be + lead and bath should be - lead
DURATION 120ms
VOLTAGE 30-80V
Single cell electroporation/iontophoresis of DNA:
Do not use capacitors
Set output to MONO - pipette should be - lead and bath should be + lead
FREQUENCY 200Hz
DURATION 1ms
VOLTAGE 30-80V (too high will clog tip)
Give 3-5 bursts of about 1 sec each. (REPEAT not SINGLE button)