NASCO INJECTION SCHEDULE FOR XENOPUS BREEDING

Make up HCG solution of 500IU per 1 mL.

(in 2500IU vial add 5cc 0.9% saline)

Store HCG solution at 4C for up to three months.

Day 1:

Morning (9am) – Inject male with 150IU. Place in separate tank.

Day 2:

Morning (9am) – Inject female with 250IU. Place in breeding tank.

Inject male with 150IU. Return to separate tank.

Evening (5pm) – Inject female with 500IU. Return to breeding tank.

Place male into breeding tank with female.

Day 3: Collect lots of fertilized eggs. If no eggs, re-inject female with 500IU and re-inject male with 150IU