NASCO INJECTION SCHEDULE FOR XENOPUS BREEDING
Make up HCG solution of 500IU
per 1 mL.
(in 2500IU vial add 5cc 0.9%
saline)
Day 1:
Morning
(9am) – Inject male with
150IU. Place in separate tank.
Day 2:
Morning (9am)
– Inject female with 250IU.
Place in breeding tank.
Inject male
with 150IU. Return to separate tank.
Evening (5pm) – Inject female with 500IU. Return to breeding tank.
Place
male into breeding tank with female.
Day 3:
Collect lots of fertilized
eggs. If no eggs, re-inject female
with 500IU and
re-inject male with 150IU