NASCO INJECTION SCHEDULE FOR XENOPUS BREEDING

 

Make up HCG solution of 500IU per 1 mL.

(in 2500IU vial add 5cc 0.9% saline)

Store HCG solution at 4C for up to three months.

 

Day 1:

Morning (9am)  Inject male with 150IU. Place in separate tank.

Day 2:

              Morning (9am)  Inject female with 250IU.  Place in breeding tank.

                                       Inject male with 150IU.  Return to separate tank.

              Evening (5pm) – Inject female with 500IU.  Return to breeding tank.

                                         Place male into breeding tank with female.

Day 3:  Collect lots of fertilized eggs.  If no eggs, re-inject female with 500IU  and re-inject male with 150IU