NASCO INJECTION SCHEDULE FOR XENOPUS BREEDING

 

Make up HCG solution of 500IU per 1 mL.

(in 2500IU vial add 5cc 0.9% saline)

Store HCG solution at 4C for up to three months.

 

Day 1:

Morning (9am)  Inject male with 150IU. Place in separate tank.

Day 2:

              Morning (9am)  Inject female with 250IU.  Place in breeding tank.

                                       Inject male with 150IU.  Return to separate tank.

              Evening (5pm) Inject female with 500IU.  Return to breeding tank.

                                         Place male into breeding tank with female.

Day 3:  Collect lots of fertilized eggs.  If no eggs, re-inject female with 500IU  and re-inject male with 150IU