NASCO INJECTION SCHEDULE FOR XENOPUS BREEDING
Make up HCG solution of 500IU per 1 mL.
(in 2500IU vial add 5cc 0.9% saline)
Morning (9am) Ð Inject male with 150IU. Place in separate tank.
Morning (9am) Ð Inject female with 250IU. Place in breeding tank.
Inject male with 150IU. Return to separate tank.
Evening (5pm) Ð Inject female with 500IU. Return to breeding tank.
Place male into breeding tank with female.
Day 3: Collect lots of fertilized eggs. If no eggs, re-inject female with 500IU and re-inject male with 150IU