7/20/2004 SL
|
Weight per 10 ml (mg) |
Mw |
Final Conc. (mM) |
Cs Meth Sul |
182.40 |
228.0 |
90 |
MgCl2 |
10.17 |
203.3 |
5 |
TEA-Cl |
33.14 |
165.7 |
20 |
EGTA |
38.04 |
380.4 |
10 |
HEPES |
47.66 |
238.3 |
20 |
ATP |
11.00 |
551.1 |
2 |
GTP |
1.57 |
523.2 |
0.3 |
pH= 7.2 (7.15-7.25) (use CsOH)
Osmolarity 250 (use H2O)
|
Weight per 1 L* (mg) |
Mw |
Final Conc. |
NaCl |
6720.6 |
58.44 |
115 mM |
KCl |
298.2 |
74.55 |
4 mM |
CaCl2-2H2O |
441 |
147.00 |
3 mM |
MgCl2-6H2O |
610 |
203.30 |
3 mM |
HEPES |
1191.5 |
238.30 |
5 mM |
Glycine |
.76 |
75.10 |
10 mM |
Glucose |
1802 |
180.20 |
10 mM |
Picrotoxin** |
Add later |
|
100 mM |
pH= 7. 2 (7.15-7.25) (use NaOH,)
Osmolarity 250-255 (use H2O)
* start with 800ml then fill up to 1L
** picrotoxin: 240.8 mg in 4 ml water, aliquot in 100 ml, store at –20C