Internal Saline

7/20/2004 SL

 

 

Weight per 10 ml

(mg)

Mw

Final Conc.

(mM)

Cs Meth Sul

182.40

228.0

90

MgCl2

10.17

203.3

5

TEA-Cl

33.14

165.7

20

EGTA

38.04

380.4

10

HEPES

47.66

238.3

20

ATP

11.00

551.1

2

GTP

1.57

          523.2

  0.3

 

pH= 7.2  (7.15-7.25)                  (use CsOH)

Osmolarity 250                           (use H2O)

 

 

 

 

 

External Saline

 

 

Weight per 1 L*

(mg)

Mw

Final Conc.

NaCl

6720.6

58.44

115 mM

KCl

298.2

74.55

4 mM

CaCl2-2H2O

441

147.00

3 mM

MgCl2-6H2O

610

203.30

3 mM

HEPES

1191.5

238.30

5 mM

Glycine

.76

75.10

10 mM

Glucose

1802

180.20

10 mM

Picrotoxin**

Add later

 

100 mM

 

pH= 7. 2  (7.15-7.25)             (use NaOH,)                                                                                                           

Osmolarity 250-255               (use H2O)

 

*   start with 800ml then fill up to 1L

** picrotoxin:  240.8 mg in 4 ml water, aliquot in 100 ml, store at 20C