Internal Saline

7/20/2004 SL

	Weight per 10 ml (mg)	Mw	Final Conc. (mM)
Cs Meth Sul	182.40	228.0	90
MgCl2	10.17	203.3	5
TEA-Cl	33.14	165.7	20
EGTA	38.04	380.4	10
HEPES	47.66	238.3	20
ATP	11.00	551.1	2
GTP	1.57	523.2	0.3

pH= 7.2 (7.15-7.25) (use CsOH)

Osmolarity 250 (use H2O)

• start with 8ml then fill up to 10 ml
• Aliquot 500 ml/ tube, then store at –80C for up to 1 month.

External Saline

	Weight per 1 L* (mg)	Mw	Final Conc.
NaCl	6720.6	58.44	115 mM
KCl	298.2	74.55	4 mM
CaCl2-2H2O	441	147.00	3 mM
MgCl2-6H2O	610	203.30	3 mM
HEPES	1191.5	238.30	5 mM
Glycine	.76	75.10	10 mM
Glucose	1802	180.20	10 mM
Picrotoxin**	Add later		100 mM

pH= 7. 2 (7.15-7.25) (use NaOH,)

Osmolarity 250-255 (use H2O)

* start with 800ml then fill up to 1L

** picrotoxin: 240.8 mg in 4 ml water, aliquot in 100 ml, store at –20C