phenol/chloroform extraction

1.  add equal volume of phenol/chloroform/isoamyl alcholo (25:24:1)

2.  shake by hand

3.  spin 2 min at max speed

4.  carefully extract aqueous phase (top) containing DNA

5.  add 2.5 volumes EtOH and 0.1 volume 3M NaAc pH5.2

6.  mix

7.  freeze for > 20 min

8.  spin for 10 min in cold room.

9.  discard supernatant.