Immunofluorescence (tadpoles sectioned in Fish Gelatin)

 

 

Dry slides 30min at 37C

 

Put slides in humid chamber

 

Incubate 2 x 5min in PBS/0.3% TritonX-100

 

Incubate 1h in blocking solution (BS) 5%NGS/1%BSA/PBS/0.03% Triton (250l/slide)

 

Dilute 1.ab in BS, spin, incubate slides 2h rt (250l/slide)

 

Wash slides 4 x 10min PBS/Triton 0.3%

 

Dilute 2.ab in BS, spin, incubate slides 1h rt (250l/slide)

 

Wash slides 3 x 10min PBS

 

Mount in Mounting medium