Immunofluorescence (tadpoles sectioned in Fish Gelatin)
Dry slides 30min at 37°C
Put slides in humid chamber
Incubate 2 x 5min in PBS/0.3% TritonX-100
Incubate 1h in blocking solution (BS) 5%NGS/1%BSA/PBS/0.03% Triton (250µl/slide)
Dilute 1.ab in BS, spin, incubate slides 2h rt (250µl/slide)
Wash slides 4 x 10min PBS/Triton 0.3%
Dilute 2.ab in BS, spin, incubate slides 1h rt (250µl/slide)
Wash slides 3 x 10min PBS
Mount in Mounting medium