Immunofluorescence (tadpoles sectioned in Fish Gelatin)

Dry slides 30min at 37°C

Put slides in humid chamber

Incubate 2 x 5min in PBS/0.3% TritonX-100

Incubate 1h in blocking solution (BS) 5%NGS/1%BSA/PBS/0.03% Triton (250µl/slide)

Dilute 1.ab in BS, spin, incubate slides 2h rt (250µl/slide)

Wash slides 4 x 10min PBS/Triton 0.3%

Dilute 2.ab in BS, spin, incubate slides 1h rt (250µl/slide)

Wash slides 3 x 10min PBS

Mount in Mounting medium