Sequencing at Genome Quebec
The Sequencing
Platform provides the following standard set of common primers free of charge:
T7
5’ - TAATACGACTCACTATAGGG - 3’
T3
5’ - AATTAACCCTCACTAAAGGG - 3’
SP6
5’ - TATTTAGGTGACACTATAG - 3’
M13 forward
5’ - GTAAAACGACGGCCAGT - 3’
M13 reverse
5’ - GGAAACAGCTATGACCATG - 3’
You are required to provide all other
sequencing primer(s) at a concentration of 20 uM. Please provide aliquots of 5
ul of primer for each sample to be sequenced with that primer in a separate
tube. All unused
DNA and/or primers will be kept for a maximum of two weeks after transmission
of results. If 12 or more samples need to be sequenced with the same primer or
if your primers are in limited amount, you may provide a single tube of primer
for the sequencing of those samples. In such case there must be at least 1 ul
of p[rimer for each sequencing reaction. Make sure that the samples are placed
in a consecutive order on sample sheet (e.g. samples are positions B01 to B12).
All DNA
samples must be sent in strip tubes or 96-well plates at concentration
between 100 ng/ul and 500 ng/ul in a volume of 5-10 ul. (200uL PCR tubes are OK
too.) This
concentration is used as an approximation. You do not have to measure the
concentration. We recommend that you verify quality and quantity of DNA samples
by agarose gel electrophoresis. It is your responsibility to send templates of
good quality and in sufficient amounts.
5. Send samples by mail or in person
The samples can be
sent to us by mail at the address shown below. You can also bring the samples
in person on week days between 9 am and 5 pm.
Please send samples
to:
Pierre Lepage ou Corine Zotti
McGill University
and Genome Quebec Innovation Centre
740 Dr. Penfield
Ave
Room 7300
Montreal, QC
H3A 1A4
Tel: 514-398-3311, extension 00346# (PL)
or 00522# (CZ).
The sample
submission form for sequencing must be e-mailed to following E-mail
address: corine.zotti@mail.mcgill.ca
Check
finished sequence at https://genomequebec.mcgill.ca/nanuqAdministration/
User name: jliu12
Password: ask Ed