Solutions
100X Steinberg’s Stock
119g
Hepes
34g NaCl
2.06g
magnesium sulfate•(7 H2O)
0.8g calcium nitrate•(4 H2O)
0.5g potassium chloride
bring to 1L using milliq H2O and pH 7.5
Steinberg’s
(working solution)
100
ml 100x steinberg’s stock
500 ul pen/strep pH 7.4
500
ml steinberg’s solution
0.1g
ms222 powder pH 7.4
MBS-H (Modified Barth
solution) 10x/1l
88mM NaCl 51.4g
1mM KCl 0.75g
2.4mM NaHCO3 2.0g
0.82mM MgSO4
x7H2O 2.0g
0.33mM Ca(NO3)2
x4H2O 0.78g
0.41mM CaCl2 x2H2O 0.6g
10mM HEPES 23.8g
10mg/ml Streptomycin Sulfate and Penicillin 1ml 10mg/ml stock (-20, antibiotics)
adjust pH to 7.4 with NaOH
sterile filter buffer
NOTE: The working dilution of MBS-H is 0.1x. This means that the 10x stock should be
diluted 100x to get the final working concentration.