Solutions
100X SteinbergÕs Stock
119g
Hepes
34g NaCl
2.06g
magnesium sulfate¥(7 H2O)
0.8g calcium nitrate¥(4 H2O)
0.5g potassium chloride
bring to 1L using milliq H2O and pH 7.5
SteinbergÕs
(working solution)
100
ml 100x steinbergÕs stock
500 ul pen/strep pH 7.4
500
ml steinbergÕs solution
0.1g
ms222 powder pH 7.4
MBS-H (Modified Barth
solution) 10x/1l
88mM NaCl 51.4g
1mM KCl 0.75g
2.4mM NaHCO3 2.0g
0.82mM MgSO4
x7H2O 2.0g
0.33mM Ca(NO3)2
x4H2O 0.78g
0.41mM CaCl2 x2H2O 0.6g
10mM HEPES 23.8g
10mg/ml Streptomycin Sulfate and Penicillin 1ml 10mg/ml stock (-20, antibiotics)
adjust pH to 7.4 with NaOH
sterile filter buffer
NOTE: The working dilution of MBS-H is 0.1x. This means that the 10x stock should be
diluted 100x to get the final working concentration.