Solutions

 

100X SteinbergÕs Stock

119g Hepes

34g   NaCl

2.06g magnesium sulfate¥(7 H2O)

0.8g  calcium nitrate¥(4 H2O)

0.5g  potassium chloride    

bring to 1L using milliq H2O and pH 7.5

 

 

SteinbergÕs (working solution)

10 L  milliq water

100 ml 100x steinbergÕs stock

500 ul pen/strep    pH 7.4

 

MS222

500 ml steinbergÕs solution

0.1g ms222 powder       pH 7.4

 

Xenopus buffer

MBS-H (Modified Barth solution)                         10x/1l

88mM NaCl                                                                51.4g

1mM KCl                                                                   0.75g

2.4mM NaHCO3                                                                                     2.0g

0.82mM MgSO4 x7H2O                                             2.0g

0.33mM Ca(NO3)2 x4H2O                                          0.78g

0.41mM CaCl2 x2H2O                                                0.6g

10mM HEPES                                                            23.8g

10mg/ml Streptomycin Sulfate and Penicillin              1ml 10mg/ml stock (-20, antibiotics)

 

adjust pH to 7.4 with NaOH

sterile filter buffer

 

NOTE:  The working dilution of MBS-H is 0.1x.  This means that the 10x stock should be diluted 100x to get the final working concentration.